2B-1 Introduction to Chromatography.pdf

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Learning Objectives
1
Apply the principles of chromatography
Describe the methods used
in the analysis of compounds
Explain the chemistry behind the
methods of analysis of compounds
Identify which method is more effective
for the analysis of compounds
2
3
4
1
Analysis of Compounds
Analysis of
1
2
3
4
Fibres
Dyes
Cotton, wool, silk, polyester, nylon?
Athletes’ blood / urine
Suspected illegal drugs
Unauthorised
substances?
2
Analysis of Compounds
Complication
1
Complex mixtures
A few or one compound(s) of interest
2
3
Analysis of Compounds
1
How to separate out mixtures
Find compound(s) of interest
2
Identify the compound
What is it?
4
Chromatography
Separate a mixture into its components
Stationary phase
Mobile phase
Inert absorbent material
Liquid or gas
5

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